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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in many tumors, which ends up in chromosome instability, and therefore could add to tumorigenesis. This protein was discovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two more splice variants had been claimed but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope recognized with the antibody.
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This guideline outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This manual outlines more attainable optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and capabilities like a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is also degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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